The incident happened on a busy road with hundreds of drivers on the way to work in the road.
The train was stopped on the tracks as the fire department determine if any passengers were injured.
At least 10 people were pictured being taken to the local hospital.
The horror smash occured in Antelope Valley while the train was on route to Los Angeles.
While travelling at high-speed the train struck a box truck on the tracks near Sunland Boulevard.
The Metrolink train, consists of six cars, hit the vehicle at around 10.40am local time.
Nearby roads will be shut down while officials investigate.
No serious injuries have been reported.